Analysis Type: Long Read Sequencing

Long-Read Sequencing Analysis for PacBio & ONT

Unlock the full potential of long-read sequencing. From structural variant detection and phasing to base modification visualization, VarSeq provides the most comprehensive toolkit for PacBio HiFi and Oxford Nanopore data analysis.

PacBio HiFi Support
Oxford Nanopore (ONT)
SV & Phasing Phasing

Overcoming the Limitations of Short-Reads

While short-read sequencing excels at SNV detection, long-read technology provides the structural context necessary to resolve complex genomic regions, repetitive sequences, and large-scale structural variants.

1

Structural Variant (SV) Detection

Identify large deletions, duplications, inversions, and translocations with high precision. Long-reads allow for direct observation of breakpoints, resolving variants that are invisible to short-read read-depth algorithms.

CNV & SV Analysis
2

Haplotyping & Phasing

Resolve compound heterozygosity and determine if variants are in-cis or in-trans. Long-reads provide long-range phasing information, essential for clinical diagnostics in rare disease and pharmacogenomics.

3

Base Modification Visualization

Directly visualize 5mC methylation and other base modifications in GenomeBrowse. Gain early insight into epigenetic markers alongside primary sequence data without bisulfite treatment.

Visualize Large Alignments
Phased Alignment Visualization
Haplotype-Aware View
Haplotype 1Phased (PS: 1242)
Haplotype 2Phased (PS: 1242)
Compound Heterozygous Detected

Phasing data confirms that two pathogenic variants are located on opposite alleles (in-trans), supporting a clinical diagnosis of an autosomal recessive condition.

One Platform for Short and Long Reads

Labs don't need to choose one technology — they need a platform that handles both. VarSeq provides a single environment for short-read targeted panels and long-read whole genomes, so you can use the right sequencing approach for each clinical question.

Targeted Panels on Short Reads

Keep using cost-effective short-read panels for hereditary cancer, cardiac, and other validated assays where deep coverage and proven accuracy matter most.

Long Reads for Tricky Genes

Use long reads where short reads fall short: SMN1/SMN2 copy number and conversion, CYP2D6 star-allele phasing, and other complex pharmacogenomic loci that require long-range haplotype resolution.

Long-Read Whole Genomes

Run full WGS on PacBio HiFi or ONT for comprehensive SV detection, genome-wide phasing, and base modification analysis — all interpreted within the same VarSeq platform your team already knows.

Targeted Short Reads

Validated clinical panels with deep coverage for SNVs, indels, and read-depth CNV calling.

Long-Read WGS

PacBio HiFi & ONT for SVs, phasing, methylation, and complex gene resolution.

Same VarSeq Platform
Any Sequencer, Any Read Length

One set of filter workflows, ACMG classification tools, and clinical reports — regardless of whether the input is Illumina, PacBio, or ONT.

Long-Read Insights & Webcasts

Expert insights and webcasts on the latest in long-read sequencing technology.

Ready to Implement Long-Read Sequencing?

Join the clinical laboratories and research centers worldwide that trust Golden Helix for their advanced sequencing analysis workflows.

PacBio & ONT Ready
Phased SV Detection
ISO 13485 Certified QMS