VarSeq Suite: Variant Interpretation & Analysis

Filter, Annotate & Prioritize Variants

From raw VCF to clinically actionable findings, VarSeq delivers an integrated variant analysis workflow with real-time filter chains, curated annotation sources, and powerful prioritization across panels, exomes, and genomes.

ISO 13485 Certified QMS
CE Marked (IVDR)

Integrated Variant Analysis at Every Scale

VarSeq combines curated annotation, real-time filter chains, and phenotype-driven prioritization into a single platform. No scripting required—from gene panels to whole genomes.

Curated Annotation

Automatically annotate variants at import with ClinVar, gnomAD, OMIM, COSMIC, SIFT, PolyPhen2, and more. Monthly curated updates keep your knowledge current without external dependencies.

Intelligent Filter Chains

Build real-time filter chains with complex nested logic (AND, OR, NOT) combining population frequency, inheritance patterns, functional impact, and quality metrics. Rapidly refine results without rerunning pipelines.

Phenotype-Driven Prioritization

Rank variants by clinical relevance using HPO and OMIM biomedical ontologies. PhoRank algorithm surfaces the most phenotypically relevant candidates, dramatically reducing review time for exomes and genomes.

From VCF to Clinical Action

VarSeq's end-to-end pipeline connects every step of variant analysis into a single reproducible, auditable workflow.

5
Steps from import to report
1

Import & Annotate

Import VCF files and automatically annotate against curated databases: ClinVar, gnomAD, OMIM, COSMIC, and functional predictors like SIFT and PolyPhen2.

2

Filter & Prioritize

Apply real-time filter chains with nested logic: population frequency, inheritance mode (de novo, compound het, X-linked), functional impact, and phenotype-driven PhoRank scoring.

3

Visual Inspection

Verify candidates in GenomeBrowse with BAM pileup, coverage tracks, ClinVar overlays, and transcript context. Inspect read-level evidence to confirm variant calls.

4

Classify (VSClinical)

Evaluate variants in VSClinical with automated ACMG criteria scoring, probabilistic pathogenicity assessment, and integrated evidence from population data and functional predictions.

5

Report & Archive

Generate clinical reports integrating variants, classifications, evidence, and quality metrics. Archive results in VSWarehouse for longitudinal tracking and reanalysis.

Annotation Sources

ClinVarClassified variants with review stars and lab interpretations
gnomADGlobal allele frequencies with sub-population breakdown
OMIMGene-disease associations and inheritance patterns
COSMICSomatic mutation hotspots for oncology workflows
SIFT / PP2Functional impact predictions and conservation scores
Monthly Updates
Local Storage

The Annotation Advantage

Consistent, reproducible annotation is the foundation of clinical variant analysis. VarSeq stores all annotation data locally and updates it monthly, so your results never depend on external service availability.

  • Full HGVS notation (g., c., p.) across all overlapping transcripts
  • Automatic right-shifting of indels to correct 3' coding position
  • Gene constraint metrics (missense z-scores) for pathogenicity assessment
  • Conservation scoring across 100 vertebrates (GERP++, PhyloP)

See VarSeq in Action

Request a personalized demo of the variant analysis workflow.

Request Evaluation

Filter Chains That Find What Matters

VarSeq's real-time filter chain engine supports every clinical scenario with full nested logic (AND, OR, NOT). Analyze family-based inheritance, cohort comparisons, and rapid iterative exploration across all imported and annotated data.

Inheritance Filtering

Full pedigree support: de novo, autosomal dominant and recessive, X-linked, compound heterozygous, and custom sample groupings for cohort analysis.

"97.3% of pathogenic variants have an allele frequency below 0.01% in gnomAD—population frequency filtering is the single most powerful first step."

Filter Categories

Population Frequency

gnomAD, 1000 Genomes with configurable AF thresholds and sub-population filtering.

Inheritance Patterns

De novo, autosomal dominant/recessive, X-linked, compound het. Full pedigree with trio/quad support.

Functional Impact

Loss-of-function prioritization, missense constraint, splice-site impact, and sequence ontology terms.

Phenotype & Coverage

HPO-driven PhoRank scoring, coverage quality thresholds, and regional BED file analysis.

Variant Analysis Insights & Webcasts

Deep dives into variant filtering strategies, annotation best practices, and clinical analysis workflows.

On-Demand Webcasts

View All Webcasts

Ready to Transform Your Variant Analysis?

Join leading clinical laboratories worldwide using VarSeq for integrated variant filtering, annotation, and interpretation.

Predictable Pricing
Data Sovereignty
ISO 13485-Certified QMS